Application

Analytes: sCD137, Eotaxin/CCL11, sFasL, FGF-2, Fractalkine/CX3CL1, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10/CXCL10, MIP-3α/CCL20, Perforin, RANTES/CCL5, TNFβ/LTA Recommended Sample type: Serum, plasma, or cell culture supernatant Recommended Sample dilution: 1:2 diluted plasma or serum NOTE: RANTES/CCL5 is for tissue culture samples only and cannot be combined with other analytes in this panel when measuring serum or plasma due to different dilution needs Assay Run Time: Overnight or one day. For best results, an overnight incubation is recommended Research Category: Inflammation & Immunology

Components

Non-Human Primate Cytokine Panel 2 Standard, 1 vial, lyophilizedNon-Human Primate Cytokine Panel 2 Quality Control 1, 1 vial, lyophilizedNon-Human Primate Cytokine Panel 2 Quality Control 2, 1 vial, lyophilizedSerum Matrix (0.08% Sodium Azide), 1 bottle, lyophilizedSample Plate, 96 well format, 1 plate2 Foil plate sealersAssay Buffer, 2 bottles, 30 mLBead Diluent, 1 bottle, 3.5 mLWash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mLNon-Human Primate Cytokine Panel 2 Detection Antibodies, 1 bottle, 3.2 mLStreptavidin-Phycoerythrin, 1 bottle, 3.2 mLMixing Bottle, 1 bottle

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Cytokines, immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses, are one of the regulatory mechanisms of the immune system. Acting at the recognition, activation or effector phases of an immune response, cytokines modulate the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Since chronic inflammation and aberrant immune response contribute to many disease states, research involving cytokines and chemokines plays a significant role in achieving a deeper understanding of the etiology and progression of diseases such as infectious disease, autoimmune and chronic inflammatory disease, cardiovascular disease, metabolic syndrome, neurological disorders and cancer. The MILLIPLEX^® Non-Human Primate Cytokine Panel II is a 25 plex kit to be used for the simultaneous quantification of any or all of the following analytes in monkey serum, plasma, tissue/cell lysate and culture supernatant samples: sCD137, Eotaxin, sFASL, FGF-2, Fractalkine, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10, MIP-3α, Perforin, RANTES and TNFβ. The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Cytokines/Chemokines

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Please note: Sample Type must be selected when configuring this kit. RANTES/CCL5 is for tissue culture samples only and cannot be combined with other analytes for serum/plasma.

Sensitivity: Refer to kit protocol for sensitivities for individual cytokines/chemokines

Please contact Technical Service for linearity of dilution.