Application

Analytes: Erythropoietin (EPO), Exodus-2, Fractalkine/CX3CL1, IFNβ1, IFNγ, IL-11, IL-16, IL-17A/F, IL-20, MCP-5/CCL12, MDC (CCL22), MIP-3α/CCL20, MIP-3β/CCL19, TARC/CCL17, TIMP-1 Recommended Sample type: serum, plasma, and cell culture Recommended Sample dilution: 1:2 dilution of serum and plasma. Tissue culture supernatant may require a dilution with an appropriate control medium Assay Run Time: overnight or one day Research Category: Inflammation & Immunology

Components

Mouse Cytokine/Chemokine Panel II Standard, 1 vial, lyophilizedMouse Cytokine/Chemokine Panel II Quality Control 1, 1 vial, lyophilizedMouse Cytokine/Chemokine Panel II Quality Control 2, 1 vial, lyophilizedSerum Matrix (0.08% Sodium Azide), 1 vial, lyophylizedAssay Buffer, 1 bottle, 30 mLWash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL eaMouse Cytokine/Chemokine Panel II Detection Antibodies, 1 bottle, 3.2 mLMouse Cytokine/Chemokine Panel II Antibody-Immobilized Beads (50X), 90 µL eaStreptavidin-Phycoerythrin, 1 bottle, 3.2 mLMixing Bottle, 1 bottleSample Plate, 96 well format, 1 plate2 Foil Plate Sealers

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. In addition, the mouse model and mouse-to-human translational studies provide data essential to understanding the role of cytokines and immune response in both normal and disease states. The MILLIPLEX^® Mouse Cytokine/Chemokine Bead Panel II, contains all the components necessary to measure the following 15 biomarkers in any combination using Luminex^® xMAP^® technology: Erythropoietin (EPO), Exodus-2/CCL21/6Ckine, Fractalkine/CX3CL1, IFNβ1, IFNγ, IL-11, IL-16, IL-17A/F, IL-20, MCP-5/CCL12, MDC/CCL22, MIP-3α/CCL20, MIP-3β/CCL19, TARC /CCL17, TIMP-1. The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 serum, plasma, or cell/tissue culture samples in duplicate. Panel Type: Cytokines/Chemokines

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual cytokines/chemokines.

Please contact Technical Service for linearity of dilution.

Packaging

Everything you need in a single kit.

Specificity

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.