Application

Analytes: Adiponectin, IL-6, Leptin, MCP-1, PAI-1 (Total), Resistin, TNFα Recommended Sample Type: Mouse tissue culture supernatants Recommended Sample Dilution: 10 µL per well of tissue culture supernatant; dilution with an appropriate control medium may be required. Assay Run Time: Overnight (16-18 hours) at 2-8°C or 2 hours at room temperature (20-25°C). Research Category: Metabolism Research Subcategory: Endocrine, Obesity, Metabolic Disorders

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

The role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of leptin secretion from adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. One of these adipokines, adiponectin, is involved in the regulation of lipid and glucose metabolism, influencing the body’;s response to insulin. Its anti-inflammatory effects on the cellular lining of blood vessel walls may explain the association of high adiponectin levels with a reduced risk of heart attack. MILLIPLEX^® Mouse Adipocyte Panel is a 7-plex kits used for the simultaneous quantification of any or all of the following analytes in mouse tissue culture supernatant samples: Adiponectin, IL-6, Leptin, MCP-1, PAI-1 Total, Resistin and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Metabolism

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sensitivity: Please see kit protocol for sensitive's of individual biomarkers.