Application

Analytes: Cortisol, Melatonin Recommended Sample type: serum, plasma, cerebrospinal fluid (CSF), tissue/cell lysate, and culture supernatant samples NOTE: Sample extraction protocols are required for serum/plasma samples. Other sample types (e.g. CSF, culture supernatant, saliva, etc.) do not require sample extraction. Recommended Sample dilution: 1:4 for extracted serum and plasma or neat for CSF and culture supernatant Assay Run Time: Overnight Research Category: Neuroscience Metabolism Research Subcategory: Category Metabolic Disorders Obesity Inflammation & Autoimmune Mechanisms NOTE: This is a competitive assay

Components

Human Circadian/Stress Standard, 1 vial, lyophilizedHuman Circadian/Stress Quality Control 1, 1 vial, lyophilizedHuman Circadian/Stress Quality Control 2, 1 vial, lyophilizedAssay Buffer, 2 bottles, 30 mL eaWash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL eaHuman Circadian/Stress Primary Antibodies, 1 bottle, 3.5 mLHuman Circadian/Stress Detection Antibodies, 1 bottle, 5.5 mLHuman Circadian/Stress Antibody-Immobilized Magnetic Beads, 20X, 200 uL eaStreptavidin-Phycoerythrin, 1 bottle, 5.5 mLMixing Bottle, 1 bottleSample Plate, 96 well format, 1 plate with 2 Foil Plate Sealers

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

The MILLIPLEX^® Human Circadian/Stress Bead Panel enables you to focus on the relevance and therapeutic potential of Melatonin and Cortisol in your research model. Coupled with the Luminex^® xMAP^® platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity. The MILLIPLEX^® Human Circadian/Stress Bead Panel is to be used for the simultaneous quantification of Cortisol and Melatonin. This is a configurable assay, and either or both analytes can be selected. This kit may be used for the analysis of human serum, plasma, tissue/cell lysate, culture supernatant samples, or cerebrospinal fluid (CSF). Serum or plasma samples should be extracted using either the acetonitrile precipitation technique or the Waters 96-well HLB extraction plate. Please refer to the protocol for more information on both methods. The necessary components to perform the extractions are not included with this kit. Panel Type: Neuroscience

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

96-well plate

Everything you need in a single kit.

Specificity

Cross ReactivtyThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.