Milliplex Catalog ID.HMYOMAG-56K-13.Huma

Code: HMYOMAG-56K-13 D2-231

Application

Analytes: Apelin (APLN), Brain-derived Neurotrophic Factor (BDNF), Erythropoeitin (EPO), Fatty Acid-Binding Protein 3 (FABP3), Fibroblast Growth Factor 21 (FGF21)...


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Application

Analytes: Apelin (APLN), Brain-derived Neurotrophic Factor (BDNF), Erythropoeitin (EPO), Fatty Acid-Binding Protein 3 (FABP3), Fibroblast Growth Factor 21 (FGF21), Fractalkine (CX3CL1), Follistatin-Like 1 Protein (FSTL-1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN/GDF8), Oncostatin M (OSM), Osteocrin (OSTN/Musclin), Osteonectin (SPARC) Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or lysates NOTE: Substantial amounts of BDNF are stored in circulating platelets and subsequently released upon platelet activation. Therefore, platelet-poor plasma is critical to ensure accurate measurement of circulating levels of BDNF. Recommended Sample Dilution: 25 µL per well of 1:2 diluted serum or plasma. Cell/tissue culture samples may be run neat or appropriately diluted in control media. Assay Run Time: Overnight (16-18 hours) at 2-8°C or 2 hours at room temperature (20-25°C) Research Category: Bone Research Subcategory: Metabolism

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Muscle contraction during physical activity is an important activator of the release of the myokines. Myokines not only act in an autocrine and/or paracrine manner in regulation of skeletal muscle growth, but they also act as endocrine hormones to mediate inter-organ crosstalk. The myokine theory provides molecular mechanistic explanations for exercise-induced metabolic changes in liver and adipose tissue and for the profound changes found in immune and neuronal systems. MILLIPLEX® Human Myokine Panel is a15-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum,plasma and cell/tissue supernatant samples: Fractalkine/CX3CL1, BDNF, Oncostatin M (OSM), Osteonectin (SPARC), LIF, FGF-21, Erythropoietin (EPO), IL-15, IL-6, Apelin, Myostatin/GDF8, Irisin, Osteocrin/Musclin, FABP3 and Follistatin-Like Protein (FSTL 1). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Metabolism

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Please contact Technical Service for linearity of dilution.

assay rangesensitivity: 5 pg/mL(MinDC+2SD; IL-15), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(Fractalkine/CX3CL1), standard curve range: 2-10,000 pg/mL(IL-15), sensitivity: 815 pg/mL(MinDC+2SD; Erythropoietin (EPO)), sensitivity: 9 pg/mL(MinDC+2SD; BDNF), sensitivity: 9.8 pg/mL(MinDC+2SD; FABP3), inter-assay cv: ﹤20%intra-assay cv: ﹤10%(Osteonectin (SPARC)), accuracy: 100%(Apelin), accuracy: 97%(LIF), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(FABP3), standard curve range: 49-200,000 pg/mL(Fractalkine/CX3CL1), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(Irisin), standard curve range: 2-10,000 pg/mL(BDNF), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(FGF-21), accuracy: 92%(IL-15), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(Erythropoietin (EPO)), sensitivity: 7.5 ng/mL(MinDC+2SD; Osteonectin (SPARC)), sensitivity: 59 pg/mL(MinDC+2SD; Osteocrin/Musclin), sensitivity: 259 pg/mL(MinDC+2SD; Myostatin/GDF8), sensitivity: 4 pg/mL(MinDC+2SD; LIF), standard curve range: 122-500,000 pg/mL(Apelin), sensitivity: 6 pg/mL(MinDC+2SD; Oncostatin M (OSM)), standard curve range: 10-40,000 pg/mL(LIF), standard curve range: 7-30,000 ng/mL(Osteonectin (SPARC)), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(Oncostatin M (OSM)), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(IL-6), accuracy: 98%(Oncostatin M (OSM)), accuracy: 96%(FSTL-1), standard curve range: 5-20,000 pg/mL(FGF-21), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(LIF), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(Apelin), standard curve range: 2-6,000 pg/mL(Oncostatin M (OSM)), standard curve range: 49-200,000 pg/mL(Osteocrin/Musclin), standard curve range: 244-1,000,000 pg/mL(Irisin), standard curve range: 24-100,000 pg/mL(FABP3), sensitivity: 0.9 pg/mL(MinDC+2SD; IL-6), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(Follistatin-like Protein 1 (FSTL1)), sensitivity: 281 pg/mL(MinDC+2SD; Irisin), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(IL-15), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(Osteocrin/Musclin), standard curve range: 488-2,000,000 pg/mL(Follistatin-like Protein 1 (FSTL1)), standard curve range: 244-1,000,000 pg/mL(Erythropoietin (EPO)), standard curve range: 0.6-2,400 pg/mL(IL-6), sensitivity: 14 pg/mL(MinDC+2SD; FGF-21), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(Myostatin/GDF8), sensitivity: 40 pg/mL(MinDC+2SD; Fractalkine/CX3CL1), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(BDNF), standard curve range: 488-2,000,000 pg/mL(Myostatin/GDF8), sensitivity: 737 pg/mL(MinDC+2SD; Follistatin-like Protein 1 (FSTL1)), sensitivity: 64 pg/mL(MinDC+2SD; Apelin)
detection methodfluorometric (Luminex xMAP)
manufacturer/tradenameMilliplex®
Quality Level200
shipped inwet ice
species reactivityhuman
technique(s)multiplexing: suitable
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