Application

Analytes: MMP-3, MMP-12, MMP-13 Recommended Sample type: Serum and plasma; this panel has also been analytically verified in urine, tissue/cell lystate and culture supernatant samples Recommended Sample dilution: Neat Assay Run Time: One day Research Category: Inflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

MMPs (matrix metalloproteinases), a family of zinc proteases responsible for the breakdown of extracellular matrix (ECM), play a key role in normal physiological processes, such as embryonic development and tissue morphogenesis, tissue and bone remodeling, wound healing, and angiogenesis. These processes rely on MMPs’; role in the cleavage of cell surface receptors, the release of apoptotic ligands, cell proliferation and differentiation, and chemokine activity modulation. Similar in structure, MMPs are synthesized and secreted as inactive pro-enzymes that require proteolytic cleavage for activation. This process can be mediated by serine proteases or other MMPs. An increase in MMP expression occurs in response to a wide range of stimuli, including adhesion molecules, growth factors, cytokines, and hormones. Regulation of MMP activity is controlled primarily by TIMPs (tissue inhibitors of metalloproteinases). Therefore, disruption of the MMP/TIMP balance can result in arthritis, cardiovascular disease and tumor growth and metastasis. MILLIPLEX^® Human MMP Bead Panel 1 is a 3 plex kit to be used for the simultaneous quantification of any or all the following analytes in serum/plasma samples: MMP-3, MMP-12, and MMP-13. The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Cytokines/Chemokines

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sensitivity: See protocol for sensitivities of individual analytes

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.