Application

Analytes: α-Fetoprotein (AFP), ANGPTL3, ANGPTL4, ANGPTL6 (AGF), FABP1 (L-FABP), FGF-19, FGF-21, FGF-23, HGF Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or lysates NOTE: Serum samples are recommended when measuring ANGPTL6/AGF Recommended Sample Dilution: 12.5 µL per well of 1:2 diluted serum or plasma. Cell/tissue culture samples may be used undiluted or appropriately diluted in control medium. Assay Run Time: Overnight (16-18 hours) at 2-8°C is recommended for optimal sensitivity. 2 hours at room temperature (20-25° C) may also be used. Research Category: Metabolism Research Subcategory: Metabolic Disorders

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Liver-secreted proteins play important roles in metabolic homeostasis through regulation of circulating lipoprotein levels, energy expenditure, glucose metabolism, and fatty acid uptake. Liver-secreted proteins may serve as biomarkers for liver diseases and gastric cancer. Accurate measurement of liver proteins is critical to obtaining an understanding of their biological functions. MILLIPLEX^® Human Liver Protein Panel is a 9-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue/cell culture samples: AFP, ANGPTL3, ANGPTL4, ANGPTL6/AGF, FABP1/L-FABP, FGF-19, FGF-21, FGF-23 and HGF. NOTE: When assaying ANGPTL6/AGF it is recommended that serum samples be used. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Metabolism

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Please contact Technical Service for linearity of dilution.

Packaging

96 well plate

Everything you need in a single kit.

Specificity

Cross ReactivtyThe ANGPTL6 standard had cross reactivity with the ANGPTL4 assay of less than 2%. For all other analytes, there was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.