Application

Analytes: IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGFBP-7 Recommended Sample Type: Human serum, plasma, and cell culture conditioned-media Recommended Sample Dilution: For serum and plasma samples, 25 µL per well of a 1:25 dilution should be used when IGFBP3 is not included in the assay. Use a 1:200 dilution when IGFBP3 is included in the assay. Tissue culture supernatants may be run undiluted or appropriately diluted with control medium. Assay Run Time: Overnight (16-20 hours) at 2-8°C. For improved sensitivity for some analytes use a 2 hour incubation at room temperature (20-25°C). Research Category: Metabolism Research Subcategory: Metabolic Disorders

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Insulin-like growth factor (IGF) binding proteins (IGFBP) are expressed by multiple tissues and cells, including hepatic cells. By binding both IGF-1 and IGF-2, IGFBPs play a critical role in the regulation of IGF action, altering IGF interaction with cell surface receptors and increasing circulating IGF half-life and bioavailability. While their functions at the cellular level are not fully understood, studies have demonstrated the capacity to both stimulate and inhibit IGF-1 activity, depending on the system involved and the IGFBP studied. The ability of IGFBPs to regulate cell sensitivity by inhibiting IGF signaling may function in the control of tumor cell growth. The MILLIPLEX^® Human IGF Binding Protein (IGFBP) Panel, is used for the simultaneous quantification of any or all of the following analytes in human serum, plasma, and cell culture conditioned-media samples: IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, and IGFBP-7. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Endocrine

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Please contact Technical Service for linearity of dilution.

Packaging

96 well plate

Everything you need in a single kit.

Specificity

Cross ReactivtyThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.