Application

Analytes: Angiostatin, soluble AXL (sAXL) , soluble c-Kit/Stem Cell Growth Factor Receptor (sc-Kit/SCFR), soluble E-Selectin (sE-Selectin), soluble Epidermal Growth Factor Receptor (sEGFR/sErbB1/sHER1)†, soluble Human Epidermal Growth Factor Receptor 2 (sHer2/sHER2/sErbB2), soluble Human Epidermal Growth Factor Receptor 3 (sHer3/ sHER3 / sErbB3), soluble Hepatocyte Growth Factor Receptor (sHGFR/c-Met/sc-Met), soluble IL-6Rα (IL-6Rα (soluble)/sIL-6Rα), soluble Neuropilin-1 (sNRP-1), Osteopontin (OPN), Platelet Derived Growth Factor-AB/BB (PDGF-AB/BB), soluble Platelet Endothelial Cell Adhesion Molecule-1 (sPECAM-1/sCD31), Thrombospondin-2 (TSP-2), soluble Tie-2 (sTie-2), soluble Urokinase-type Plasminogen Activator Receptor (suPAR), soluble Vascular Endothelial Growth Factor Receptor 1 (soluble VEGFR1, sVEGFR1/ sFlt-1), soluble Vascular Endothelial Growth Factor Receptor 2 (soluble VEGFR2/sVEGFR2/ sKDR/sFlk-1), soluble Vascular Endothelial Growth Factor Receptor 3 (soluble VEGFR3/sVEGFR3/ sFlt-4). †Antibody against active site results in preferential binding to unbound EGFR. Recommended Sample Type: Human serum, plasma, cell culture supernatants and tissue/cell homogenates/lysates Recommended Sample Dilution: 25 µL per well of 1:5 diluted plasma or serum; cell culture supernatant may be used undiluted or diluted with appropriate control medium Assay Run Time: Overnight (16-18 hours) at 2-8°C Research Category: Cancer

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Angiogenesis, the development of new vascular networks, is a key process in normal growth and development as well as in wound healing, with homeostasis maintained by a delicate balance of angiogenic factors and inhibitors. Consequently, insufficient, or excessive blood vessel growth underlies many diseases, including cardiovascular disease, diabetic ulcers, macular degeneration, and cancer. Angiogenesis plays a significant role in tumor growth and metastasis. MILLIPLEX^® Human Angiogenesis Magnetic Bead Panel 2 is a 20-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, cell culture samples, and tissue homogenates/lysates: Angiostatin, soluble E-Selectin, (sE-Selectin), Osteopontin (OPN), Platelet Derived Growth Factor-AB/BB (PDGF-AB/BB), soluble Platelet Endothelial Cell Adhesion Molecule-1 (sPECAM-1), Thrombospondin-2 (TSP-2), soluble AXL (sAXL), soluble c-Kit/Stem Cell Growth Factor Receptor (sc-Kit/SCFR), soluble Epidermal Growth Factor Receptor (sEGFR)†, soluble Human Epidermal Growth Factor Receptor 2 (sHer2), soluble Human Epidermal Growth Factor Receptor 3 (sHer3), soluble Hepatocyte Growth Factor Receptor/c-Met (sHGFR/c-Met), soluble IL-6Rα, soluble Neuropilin-1 (sNRP-1), soluble Tie-2 (sTie-2), soluble Urokinase-type Plasminogen Activator Receptor (suPAR), soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR1), soluble Vascular Endothelial Growth Factor Receptor 2 (sVEGFR2), and soluble Vascular Endothelial Growth Factor Receptor 3 (sVEGFR3). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. †Antibody against active site results in preferential binding to unbound EGFR. The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Circulating Cancer

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sensitivity: See kit protocol for individual analytes sensitivities.

Please contact Technical Service for linearity of dilution.