Cat ID.HADK1MAG-61K-03.Human Adipokine

Code: HADK1MAG-61K-03 D2-231

Application

Analytes: Adiponectin Adipsin Lipocalin-2/NGAL PAI-1 (Total) Resistin Recommended Sample Type: Human serum and plasma Recommended Sample Dilution: 25 µL per ...


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Application

Analytes: Adiponectin Adipsin Lipocalin-2/NGAL PAI-1 (Total) Resistin Recommended Sample Type: Human serum and plasma Recommended Sample Dilution: 25 µL per well of 1:400 diluted serum or plasma Assay Run Time: Overnight (16-18 hours) at 2-8°C Research Category: Metabolism Research Subcategory: Endocrine

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

The role of white adipose tissue was once thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. The Adipokine Adiponectin is involved in the regulation of lipid and glucose metabolism, influencing the body’;s response to insulin. Adiponectin also has anti-inflammatory effects on the cellular lining of blood vessel walls which may help explain the association of high levels with reduced risk of heart attack. The MILLIPLEX® Human Adipokine Panel 1 is a 5-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: Adiponectin, Adipsin, Lipocalin-2/NGAL, Total PAI-1, and Resistin. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Metabolism

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Please contact Technical Service for linearity of dilution.

assay rangeinter-assay cv: ﹤10%intra-assay cv: ﹤20%(Resistin), sensitivity: 21 pg/mL(MinDC+2SD; Adiponectin), inter-assay cv: ﹤10%intra-assay cv: ﹤15%(Lipocalin-2/NGAL), standard curve range: 3.2-50,000 pg/mL(Lipocalin-2/NGAL), standard curve range: 9.6-150,000 pg/mL(PAI-1 (total)), inter-assay cv: ﹤10%intra-assay cv: ﹤15%(Adiponectin), standard curve range: 13-200,000 pg/mL(Adipsin), sensitivity: 3.5 pg/mL(MinDC+2SD; Lipocalin-2/NGAL), accuracy: 87%(Resistin), standard curve range: 26-400,000 pg/mL(Adiponectin), sensitivity: 10 pg/mL(MinDC+2SD; Adipsin), inter-assay cv: ﹤10%intra-assay cv: ﹤10%(Adipsin), accuracy: 89%(Adiponectin), standard curve range: 6.4-100,000 pg/mL(Resistin), inter-assay cv: ﹤10%intra-assay cv: ﹤20%(PAI-1 (total)), sensitivity: 5.8 pg/mL(MinDC+2SD; PAI-1), sensitivity: 4.4 pg/mL(MinDC+2SD; Resistin)
detection methodfluorometric (Luminex xMAP)
manufacturer/tradenameMilliplex®
Quality Level200
shipped inwet ice
species reactivityhuman
technique(s)multiplexing: suitable
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