Milliplex Catalog ID.HADCYMAG-61K-10.Hum

Code: HADCYMAG-61K-10 D2-231

Application

Analytes: Adiponectin, HGF, IL-1β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (Total), Resistin, TNF-α Recommended Sample Type: Tissue/cell culture supernata...


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Application

Analytes: Adiponectin, HGF, IL-1β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (Total), Resistin, TNF-α Recommended Sample Type: Tissue/cell culture supernatants or extracts Recommended Sample Dilution: 25 µL per well of undiluted tissue/cell culture sample; samples may require dilution with an appropriate medium prior to assay Assay Run Time: Overnight (16-18 hours) at 2-8°C Research Category: Metabolism Research Subcategory: Endocrine

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

The role of white adipose tissue was once thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. One of these Adipokines, Adiponectin, is involved in the regulation of lipid and glucose metabolism, influencing the body’;s response to insulin. Adiponectin also has anti-inflammatory effects on the cellular lining of blood vessel walls which may help explain the association of high levels with reduced risk of heart attack. The MILLIPLEX® Human Adipocyte Panel is an 11-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cell/tissue culture samples: Adiponectin, HGF, IL-β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (total), Resistin, TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Metabolism

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Please contact Technical Service for linearity of dilution.

Packaging

96 well plate

Everything you need in a single kit.

Specificity

Cross ReactivtyThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

assay rangeaccuracy: 83%(PAI-1 (total)), standard curve range: 6.4-100,000 pg/mL(HGF), standard curve range: 0.51-8,000 pg/mL(IL-1β), accuracy: 99%(HGF), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(Resistin), inter-assay cv: ﹤25%intra-assay cv: ﹤20%(Leptin), standard curve range: 26-400,000 pg/mL(Adipnectin), sensitivity: 0.2 pg/mL(MinDC+2SD; NGF), inter-assay cv: ﹤20%intra-assay cv: ﹤10%(NGF), sensitivity: 15 pg/mL(MinDC+2SD; Adiponectin), standard curve range: 0.32-5,000 pg/mL(NGF), inter-assay cv: ﹤20%intra-assay cv: ﹤10%(PAI-1 (total)), sensitivity: 2.1 pg/mL(MinDC+2SD; MCP-1), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(IL-8), inter-assay cv: ﹤20%intra-assay cv: ﹤15%(IL-1β), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(MCP-1), sensitivity: 0.6 pg/mL(MinDC+2SD; IL-6), standard curve range: 0.38-6,000 pg/mL(TNFα), sensitivity: 0.2 pg/mL(MinDC+2SD; IL-8), standard curve range: 9.6-150,000 pg/mL(Leptin), sensitivity: 4.6 pg/mL(MinDC+2SD; PAI-1 (total)), sensitivity: 3.0 pg/mL(MinDC+2SD; HGF), sensitivity: 10 pg/mL(MinDC+2SD; Leptin), accuracy: 102%(TNFα), standard curve range: 0.96-150,000 pg/mL(MCP-1), accuracy: 103%(MCP-1), inter-assay cv: ﹤20%intra-assay cv: ﹤10%(TNFα), standard curve range: 6.4-100,000 pg/mL(Resistin), sensitivity: 2.0 pg/mL(MinDC+2SD; IL-1?), sensitivity: 0.2 pg/mL(MinDC+2SD; TNF?), accuracy: 100%(IL-6), standard curve range: 0.29-4,000 pg/mL(IL-8), standard curve range: 0.96-150,000 pg/mL(IL-6), inter-assay cv: ﹤20%intra-assay cv: ﹤10%(IL-6), standard curve range: 9.6-150,000 pg/mL(PAI-1), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(Adiponectin), inter-assay cv: ﹤20%intra-assay cv: ﹤10%(HGF), sensitivity: 4.4 pg/mL(MinDC+2SD; Resistin)
detection methodfluorometric (Luminex xMAP)
manufacturer/tradenameMilliplex®
Quality Level200
shipped inwet ice
species reactivityhuman
technique(s)multiplexing: suitable
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