Application

This Human Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β42 levels in Neuroscience research.

Used to detect/quantify: Amyloid β42

This assay requires 50 µl of sample and is an overnight assay.

Research CategoryNeuroscience

Research Sub CategoryAlzheimer′s Disease

Disclaimer

For research use only. Not for use in diagnostic procedures.

General description

Amyloid beta peptides have been implicated in the etiology of Alzheimer's disease. Amyloid beta 40 is the most prominent peptide and Amyloid beta 42 is the neurotoxic form. The Amyloid beta 42/40-ratio (AB ratio) has been reported as a better indicator of the Alzheimer pathology. Millipore's Human Amyloid β42 Brain ELISA kit is used for the measurement of Amyloid beta 42 in brain samples and for other tissue samples in a 96-well format. This assay is for research use only and appropriate for the in vitro detection of human Amyloid β42 peptides in brain samples from e.g. Guinea pig, transgenic hAmyloid mice and cell extracts.

Other Notes

Please contact Technical Service for linearity of dilution.

Specificity

The Amyloid β42 ELISA uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-42, which causes a high selectivity for Aβ42. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.

Storage and Stability

Components in the kit can be stored up to 2 weeks at 2-8°C