EQCYTMAG-93K-03 Equine Cytokine MAG

Code: EQCYTMAG-93K-03 D2-231

Application

Analytes: Eotaxin/CCL11, FGF-2, Fractalkine/CX3CL1, G-CSF, GM-CSF, GRO/KC, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 (p70), I...


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Application

Analytes: Eotaxin/CCL11, FGF-2, Fractalkine/CX3CL1, G-CSF, GM-CSF, GRO/KC, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-18, IP-10 (CXCL10), MCP-1, RANTES (CCL5), TNF-α Recommended Sample Type: Equine serum, plasma, tissue culture supernatant or tissue/cell extracts Recommended Sample Dilution: 25 µL per well of undiluted serum or plasma; tissue/cell culture samples may require dilution with an appropriate medium Assay Run Time: A primary incubation of 2 hours at room temperature (RT) (20-25°C), or for greater sensitivity, overnight (16-18 hours) at 2-8°C; followed by a 1 hour secondary incubation at RT Research Category: Inflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Inflammation and immune cell response are key to maintaining homeostasis. The more we understand the mechanisms of disease, the more we discover the role that inflammation plays in the etiology of disease. This is as true for horses as it is for humans. Both experience immunological diseases, such as infectious diseases, osteoarthritis, and respiratory diseases, as well as neurologic, metabolic, and cardiovascular diseases and cancer. MILLIPLEX® Equine Cytokine/Chemokine Premix Panel is a 23-plex kit used for the simultaneous quantification of any or all the following analytes in equine serum, plasma, or culture supernatant samples: Eotaxin/CCL11, FGF-2, Fractalkine/CS3CL1, G-CSF, GM-CSF, GRO, IFN, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8/CXCL8, IL-10, IL-12 (p70), IL-13, IL-17a, IL-18, IP-10, MCP-1, RANTES/CCL5 and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Cytokines/Chemokines

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

96 well plate

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

assay rangesensitivity: 1.4 pg/mL(IL-6), accuracy: 91%(Fractalkine/CS3CL1), accuracy: 93%(Eotaxin/CCL11), accuracy: 88%(RANTES/CCL5), standard curve range: 39-40,000 pg/mL(IL-13), sensitivity: 8.3 pg/mL(Eotaxin/CCL11), sensitivity: 10.6 pg/mL(IL-5), standard curve range: 6-6,000 pg/mL(GRO), standard curve range: 49-50,000 pg/mL(IL-10), sensitivity: 355 pg/mL(IL-4), standard curve range: 34-35,000 pg/mL(IL-18), standard curve range: 15-15,000 pg/mL(IL-6), accuracy: 74%(IL-17a), sensitivity: 13.5 pg/mL(IL-2), standard curve range: 457 pg/mL(Fractalkine/CS3CL1), sensitivity: 457 pg/mL(Fractalkine/CS3CL1), accuracy: 97%(GRO), sensitivity: 16.3 pg/mL(FGF-2), accuracy: 89%(IL-8/CXCL8), standard curve range: 59-60,000 pg/mL(IL-8/CXCL8), standard curve range: 59-60,000 pg/mL(IL-1α), accuracy: 86%(IL-10), sensitivity: 7.8 pg/mL(GRO), sensitivity: 15.4 pg/mL(IL-17A), accuracy: 83%(IFNγ), standard curve range: 8.3 pg/mL(Eotaxin/CCL11), sensitivity: 16.7 pg/mL(IP-10), accuracy: 85%(IL-13), sensitivity: 49.2 pg/mL(IL-12 (p70)), standard curve range: 16.3 pg/mL(FGF-2), standard curve range: 39-40,000 pg/mL(IL-12 (p70)), standard curve range: 293-300,000 pg/mL(IL-4), accuracy: 90%(MCP-1), sensitivity: 39-130 pg/mL(IL-13), sensitivity: 165 pg/mL(IFNγ), standard curve range: 5-5,000 pg/mL(RANTES/CCL5), accuracy: 78%(FGF-2), standard curve range: 29-30,000 pg/mL(IL-1β), standard curve range: 15-15,000 pg/mL(IP-10), standard curve range: 15-15,000 pg/mL(IL-2), standard curve range: 488-500,000 pg/mL(IFNγ), sensitivity: 29.3 pg/mL(IL-1β), accuracy: 83%(IL-12 (p70)), standard curve range: 340 pg/mL(G-CSF), sensitivity: 62.5 pg/mL(IL-10), accuracy: 82%(G-CSF), accuracy: 76%(IL-6), sensitivity: 3.94 pg/mL(GM-CSF), standard curve range: 342-350,000 ng/mL(MCP-1), sensitivity: 3.9 pg/mL(RANTES/CCL5), standard curve range: 5-5,000 pg/mL(GM-CSF), standard curve range: 4-4,000 pg/mL(TNFα), standard curve range: 15-15,000 pg/mL(IL-5), standard curve range: 15-15,000 pg/mL(IL-17A), sensitivity: 74.3 pg/mL(IL-8/CXCL8), sensitivity: 39.1 pg/mL(IL-1α), sensitivity: 399 ng/mL(MCP-1), sensitivity: 340 pg/mL(G-CSF), accuracy: 90%(IL-2), sensitivity: 25.3 pg/mL(IL-18), accuracy: 92%(IL-5), sensitivity: 5.2 pg/mL(TNFα)
detection methodfluorometric (Luminex xMAP)
manufacturer/tradenameMilliplex®
Quality Level200
shipped inambient
species reactivityhorse
technique(s)multiplexing: suitable
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