PAGE stands for Polyacrylamide Gel Electrophoresis and describes an analytical method in biochemistry for the separation of differently sized protein mixtures in an electric field. During PAGE, proteins migrate through a gel matrix in response to an applied electric field. Smaller proteins travel faster through the gel than larger proteins, leading to a size dependent separation. The most popular form of PAGE is SDS-PAGE. The detergent sodium dodecyl sulfate (SDS) is added for PAGE sample preparation and is also part of the buffer composition. The treatment with the harsh detergent leads to full protein denaturation and unfolding. SDS binds to hydrophobic parts of the unfolded protein and masks the intrinsic charge of the protein with its own negative charge. As a consequence, SDS-protein complexes migration is predominantly dependent on the size of the protein, allowing an estimation of its molecular weight. The FastGene® PAGE Protein System contains all the necessary equipment you need for PAGE protein analysis. The electrophoresis tank can hold a maximum of 4 gels simultaneously for electrophoretic protein separation.
- Complete set for PAGE protein analysis
- Most flexible use for 1 to 4 (pre-cast or hand-cast) gels
- Easiest gel hand-casting − no leaking!
- Glass plate holder and tube holder included
- Compatible with a wide range of pre-cast gels (e.g., FastGene® gels, Bio-Rad Mini-PROTEAN® Precast Gels or Thermo Fisher™ mini gels)
- Also runs hand-cast gels, and includes hand-cast gel set (for casting 1mm thick gels
- Also available are FastGene® hand-cast gel sets for gel preparation with 0.75mm or 1.5mm gel thickness
- For use of different gels, three different U sealings are included
Contents:
1 x inner electrophoresis chamber with electrodes and 2 side clips for gel holder1 x inner electrophoresis chamber without electrodes, with 2 side clips for gel holder1 x gel/blot chamber lid with electrodes and power cable1 x gel/blot chamber tank1 x plastic dummy cassette short1 x plastic dummy cassette long4 x U sealing strip long, for 10 x 10cm gels (e.g., Thermo Fisher™ mini gels)4 x U sealing strip short, for 10 x 8cm gels (Bio-Rad Mini-PROTEAN® Precast Gels and hand-cast gels)4 x U sealing strip FastGene®, for 10 x 8cm gels (FastGene® gels)4 x gel casting base4 x gel casting clips5 x sealing gaskets5 x gel shovels5 x comb 1mm 10 wells5 x comb 1mm 15 wells5 x glass spacer long 1mm10 x glass plates short1 x glass plate holder for holding 14 glass plates1 x tube holder for 50mL, 15mL, 1.5mL and PCR tubes
This product has met the following criteria to qualify for the following awards: