Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Components

Sigma’;s Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

General description

Sigma’;s Lambda DNA Hind III Digest contains 8 fragments from 125 – 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Other Notes

Contains 8 fragments, 125–23,130 bp

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Preparation Note

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.