Application

DNA Molecular Weight Marker VII, DIG-labeled, has been used as a size standard in Southern blot analysis for nucleic acid labeling and detection.

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.DNA Molecular Weight Marker VII, DIG-labeled is a ready-to-use solution in 10mM Tris-HCl, 1mM EDTA, pH 8.0. The fragment mixture is prepared by cleavage of SPP1 DNA with EcoRI. the size ranges from 0.081 to 8.57kbp.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Sequence

The mixture contains 17 fragments wit the following base pair lengths: 81, 359, 492, 710, 718, 992, 1164, 1482, 1515, 1882, 1953, 2799, 3639, 4899, 6106, 7427, and 8576 bp.Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.