A7431-25G Display Image

Agarose, BioReagent, for molecular biology, Wide range/Standard 3:1

Code: A7431-25G D2-231

Analysis Note

The following is a list of properties associated with our agaroses:Sulfate content - used as an indicator of purity, since sulfate is the major...


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Your Price
€300.00 25G
€369.00 inc. VAT

Analysis Note

The following is a list of properties associated with our agaroses:Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.Gel strength - the force that must be applied to a gel to cause it to fracture.Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

Specially formulated to form strong, flexible gels for separation of small (≤1 kb) PCR products, DNA and RNA. Stronger gels make gel-handling easier. Sharp resolution is produced with DNA fragments ranging from 10-1500 bp.

Packaging

25, 100 g in poly bottle

anion tracessulfate (SO42-): ≤0.15%
biological sourcealgae (marine)
EEO≤0.13
foreign activityDNase, RNase, NICKase, none detected
formpowder
gel strength≥1200 g/cm2 (4% gel)
gradefor molecular biology
InChI keyMJQHZNBUODTQTK-WKGBVCLCSA-N
InChI1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
mp≤90 °C
product lineBioReagent
Quality Level200
technique(s)electrophoresis: suitable
transition tempgel point 32.5-38 °C (4% gel)
Code
Description
Unit Size
List Price
Qty
A7431-100G
Unit:100G
List Price: €982.00
Source:List Price
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Cas Number9012-36-6
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