Analysis Note

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Application

Exonuclease I has been used to quantify the expression of 56 genes in each F2 XY gonad.

Biochem/physiol Actions

Exonuclease I (Exo I) fromE. colican degrade single-stranded DNA (ssDNA) from 3′ to 5′. It participates in various DNA repair systems, like, RecBCD-dependent homologous recombination, methyl-dependent mismatch repair and genomic tandem repeat maintenance.

Features and Benefits

Elimination of residual single-stranded DNA containing a 3′-terminus. Measuring endonucleolytic cleavage of covalently closed circular (ccc) ssDNA. Measuring DNA helicase activity.

General description

Acts specifically on single-stranded DNA degrading it processively in the 3′- to 5′-direction, producing 5′-mononucleotides.

Exonuclease I (Exo I) fromE. coliis an Mg²⁺ion dependent exonuclease.