Analysis Note

Hemolytic titer (CH₅₀ units per ml) is determined by method of Kabat and Mayer. Actual titer given on label.

Application

Complement sera human has been used:to incubate DLD-1 or SW-620 cells to quantify C5a releasein multiplex bead antibody-binding assay to assay C3din complement-dependent cytotoxicity assay

The activity of complement sera can be determined by hemolytic assays in vitro.

Complement sera human has been used: to incubate DLD-1 or SW-620 cells to quantify C5a release in multiplex bead antibody-binding assay to assay C3d in complement-dependent cytotoxicity assay

General description

Complement serum is prepared from pooled human plasma and lyophilized from the amount of serum indicated on the label.

Physical form

Lyophilized powder from indicated amount of serum