Analysis Note

C2 is depleted by immunoadsorption method as judged by a highly sensitive hemolytic assay and Ouchterlony immunodiffusion method.

Application

C2 is a critical protease for compliment activation, which supports bacterial killing via phagocytes. C2 can be used in research to explore the mechanisms by which bacterial pathogens evade detection by the compliment system. One such mechanism by the bacteria Pseudomonas aeruginosa, uses the alkaline protease (AprA) to degrade and cleave C2 which ultimately interferes with the classical and lectin pathways. Research has shown that C2 deficiency results in an increased risk of infection by Streptococcus pyogenes through impaired phaogocytosis and neutrophil dependent killing.

Biochem/physiol Actions

Heritable complement C2 deficiency can manifest as a number of severe dermatological disorders, including discoid lupus erythematosus, idiopathic atrophoderma, and dermatomyositis.

Physical form

Supplied as a solution in PBS, pH 7.2