Application

Live Cell imaging probe which reversibly fluoresces in acidic pH, such as in lysosomes and endosomes of cells. Cell Based Assays Antibody Labeling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

BioTracker Orange-NHS Live Cell pH dye is a chemical probe which reversibly fluoresces upon acidic pH. The probe is modified with N-hydroxysuccimide ester (NHS) which forms a covalent bond with primary amines just by mixing. It can be used for labeling proteins, antibodies and other molecules. For example, it can be applied for visualizing endocytosis of target protein on cell membrane by labeling antibodies that binds to the target protein. This probe binds to primary amines just by mixing. Thus it can be used to label antibodies and other proteins which have lysine residues. Its fluorescence intensity increases ~20-fold at an acidic pH.Spectral PropertiesAbsorbance (max): 544 nmEmission (max): 565 nm (Orange)

Packaging

1 mg

Physical form

Lyophilized

Storage and Stability

Store BioTracker Orange-NHS Live Cell pH Dye at -20°C, desiccate and protect from light.Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.