Application

Live cell imaging microtubule dye for imaging the cytoskeleton of living cells

Research Sub CategoryLive Cell Dye

Research CategoryCell Signaling

Live cell fluorescent imaging

Components

1) 1 vial of 50µL BioTracker 488 Green Microtubule Cytoskeleton Dye (1000X in DMSO) (CS224593)2) 1 vial of 100µL Verapamil HCL (100mM in DMSO) (CS224592)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cytoskeleton proteins such as actin and tubulin play an important role in cell structure and motility. Dysregulation of cytoskeletal proteins are also involved in cancer metastasis and disease. Taxol binds to polymerized tubulin and stabilizes microtubules, resulting in inhibition of mitosis. However, fluorescent taxol compounds like the BioTracker Microtubule Cytoskeleton dyes are less disruptive of microtubule dynamics and cell division, presumably due to lower binding affinity of the fluorescent probe compared to taxol itself.

The BioTracker Cytoskeleton Microtubule Dye is a green fluorescent cell-permeable taxol probe for imaging the microtubule cytoskeleton in live cells. It is a simple, rapid and sensitive stain that can be detected using the FITC channel. The dye is supplied with a vial of 100mM verapamil, an efflux pump inhibitor that may improve probe retention and staining in certain cell types.

Spectral Properties
Absorbance: 500nm
Emission: 515nm

Physical form

Liquid

Quality

Spectral Properties
Absorbance: 500nm
Emission: 515nm

Storage and Stability

Store BioTracker™ 488 Green Microtubule Cytoskeleton Dye at 2-8°C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.