Application

Research Sub CategoryLive Cell Dye

Live cell imaging dye for acidic cellular organelles such as lysosomes.

Live cell fluorescent imaging

Research CategoryCell Imaging

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Lysosomes are membrane-enclosed organelles that contain an array of enzymes capable of breaking down all types of biological material including proteins, nucleic acids, carbohydrates, and lipids. Lysosomes function as the digestive system of the cell, serving both to degrade material taken up from outside the cell and to digest obsolete components of the cell itself.

BioTracker Lysosome dyes are fluorescent stains for imaging lysosome localization and morphology in live cells. The dyes accumulate in the low pH environment of the lysosomes, resulting in highly specific lysosomal staining without the need for a wash step.

The BioTracker 555 UV-Excitation Red Lysosome is a red fluorogenic lysosome dye with pH-dependent fluorescence. The dye is unique among commercially available lysosome dyes in that its fluorescence in cells is activated by exposure to UV excitation. In solution, the dye shows pH-dependent fluorescence that does not require UV activation. The dye initially shows low fluorescence, but brief exposure to UV excitation from a mercury arc lamp through a DAPI filter.

Spectral Properties
Absorbance: 554nm
Emission: 583nm

Physical form

Liquid

Quality

Spectral Properties
Absorbance: 554nm
Emission: 583nm

Storage and Stability

Store BioTracker 555 UV-Excitation Red Lysosome Dye at -20°C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.