Application

Research Sub CategoryLive Cell Dye

Live cell imaging mitochondrial dye that stains the membrane of mitochondria used to detect cell viability, metabolic activity and overall cell health.

Live cell fluorescent imaging

Research CategoryCell Imaging

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mitochondria are organelles found in large numbers in most cells, in which the biochemical processes of respiration and energy production occurs. In metabolically active cells, mitochondria produce a membrane potential by maintaining a proton gradient across the inner and outer membranes. Loss of mitochondrial membrane potential is a hallmark for apoptosis and poor cell health.

BioTracker Mitochondria dyes are fluorogenic stains for staining mitochondria in live cells. The dyes are membrane permeable and become brightly fluorescent upon accumulation in the mitochondrial membrane. Staining is lost when mitochondria become depolarized during cell death, allowing monitoring of cell viability.

The BioTracker 488 Green Mitochondria Dye can be detected using the green FITC/GFP channel. Also, the green staining is not dependent on membrane potential, and can be used to stain both live and fixed cells.

Spectral Properties
Absorbance: 490nm
Emission: 523nm

Physical form

Lyophilized

Quality

Spectral Properties
Absorbance: 490nm
Emission: 523nm

Storage and Stability

Store BioTracker 488 Green Mitochondria Dye at -20°C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.