Application

Live cell imaging cell proliferation kit based on carboxyfluorescein succinimidyl ester (CFSE) used for fluorescent cell labeling in flow cytometry applications.

Research Sub CategoryLive Cell Dye

Live cell fluorescent imaging

Research CategoryCell Imaging

Components

1. 10 vials of BioTracker 488 Green CFSE Dye (50µg) (CS224588)2. 1 vials of Anhydrous DMSO (500µL) (CS224578)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

BioTracker SE Cell Proliferation Kits diffuse passively into live cells and are used for long-term cell labeling. They are initially non-fluorescent esters of amine-reactive dyes, but are converted to fluorescent dyes by intracellular esterases. The dyes then covalently react with amine groups on intracellular proteins, forming fluorescent conjugates that are retained in the cell. Immediately after staining a single, bright fluorescent population will be detected by flow cytometry. Each cell division that occurs after labeling is revealed by the appearance of a successively dimmer fluorescent peak on a flow cytometry histogram (Fig. 1). Cell proliferation dyes can be used to track cell divisions in vivo or in vitro. The staining can withstand fixation and permeabilization for subsequent immunostaining. Alternative applications of cell proliferation dyes include uniform labeling of cell cytoplasm for microscopy, or labeling cells for quantitation of cell number by microplate reader.

Spectral Properties
Absorbance: 495nm
Emission: 519nm

Physical form

Liquid

Quality

Spectral Properties
Absorbance: 495nm
Emission: 519nm

Storage and Stability

Store BioTracker 488 Green CSFE Cell Proliferation Kit -20°C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening. Ideally the 5 mM DMSO stock solution should be prepared on the day of use. Aliquots may be stored for later use, but activity may be reduced over time. The dyes should only be added to aqueous buffer immediately before staining.