Application

Complete serum-free organoid media for the expansion and long-term 3D culture of human intestinal colon organoids.Research Category: Cell Culture Stem Cell Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

3dGRO^™ Human Colon Organoid Expansion Medium is a complete ready-to-use serum-free medium for the expansion and long-term culture of human colonic organoids. The medium has been validated for use with 3dGRO^™ Human iPSC Derived Colon Organoids (Cat. No. SCC300). Colon organoids propagated in the medium express colon-specific markers including the posterior hindgut marker CDX2, a-carbonic anydrase II (CA-II), a-carbonic anhydrase IV (CA-IV), and goblet cell markers Mucin-2 and Mucin-5B.

Legal Information

3dGRO is a trademark of Merck KGaA, Darmstadt, Germany

Quality

Appearance (Color): Clear/No Particlulates (Red Liquid)Osmolality: 350-375 mOsmpH: 7.0 – 7.4Sterility Tested: No Growth/PassEndotoxin: ﹤2 EU/mLMycoplasma: NegativeFunctional Assay: Thaw and culture of human colon organoids for 2 passages.

Storage and Stability

Upon receipt, store at −20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly. Use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at −20°C until the expiry date.