Application

Optimized cryopreservation media for multiple organoids cell types.

Research CategoryCell CultureStem Cell Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Organoids are in-vitro derived 3D cell aggregates derived from primary tissue or stem cells that are capable of self-renewal, self-organization and exhibit organ functionality. Organoids address the limitations of existing 2D model systems by providing:

Similar composition and architecture to primary tissue: Organoids harbor small population of self-renewing stem cells (such as intestinal crypt stem cells) that can differentiate into cells of all major cell lineages, with similar frequency as in physiological conditions.
Relevant models of in-vivo conditions: Organoids are more biologically relevant to any model system and are amenable to manipulate niche components and gene expression.
Stable system for extended cultivation: Organoids can be cryopreserved as biobanks and expanded indefinitely by leveraging self-renewal and the differentiation capability of the stem cell populations.

Organoids can be cryopreserved using standard cell freezing techniques however freeze/thaw viability remains a challenge. The 3dGRO™ Organoid Freeze Medium is a proprietary optimized cryopreservation media for multiple organoids cell types (etc. mouse intestinal organoids, human colon and lung organoids). The media supports higher freeze/thaw viabilities compared to other commercial or homemade organoid freezing media formulations.

Legal Information

3dGRO is a trademark of Merck KGaA, Darmstadt, Germany

Quality

Appearance (color): Amber
Osmolality: 250-350 mOsm
pH: 6.8-7.1
Sterility Tested: No Growth/Pass
Bacterial & Fungal Tested: Passed

Storage and Stability

Store the 3dGRO™ Organoid Freeze Medium at -20°C. Aliquot into smaller working aliquots before use to avoid reagent contamination issues.