Cell Line Description

Primary Mouse Embryo Fibroblasts

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Traditionally, human embryonic stem cell (hESC) or induced pluripotent stem cell (iPSC) culture require mouse embryonic fibroblasts (MEF) support. This requirement makes the protocol complicated and variable. MEF conditioned medium (MEFCM) contains all the growth factors needed in hESC/iPSC culture, eliminating the routine preparation of feeder cells. It is a ready-to-use product for the feeder-free culture of human ES/IPS cells.

Each batch of EmbryoMax™ MEF Conditioned Media has been tested for hES/iPS cells pluripotency. The media requires an additional 10 ng/ml of bFGF to be added once thawed before use.

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality

Mycoplasma Testing: PASSED
Bacterial Testing: PASSED
Fungi Testing: PASSED

Storage and Stability

Store at -80C until expiration date on label.