Application

This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact [email protected].

Research Sub CategoryCell Cycle, DNA Replication & RepairChromatin BiologyHistones

Research CategoryApoptosis & CancerEpigenetics & Nuclear Function

Cell Line Description

Cancer Cells

General description

Chromatin, the higher order structure of DNA, proteins and RNA, constitutes the majority of the nucleus of eukaryotic cells. Changes in chromatin structure are the essence of many essential nuclear processes, including transcription, meiosis, mitosis, and apoptosis. The nucleosome represents the primary building block of chromatin and it comprises an octomer of four core histone proteins (H2A, H2B, H3 and H4). HeLa cells expressing green fluorescent protein fused histone H2B (H2B-GFP) have been widely used to visualize the dynamics of chromosomal architecture in living cells during various processes. In addition to studying normal mitosis, histone H2B-GFP has been utilized for imaging the distinctive clustering behavior of double minute chromosomes (DMs) in cancer cells during mitosis, which contributes to their asymmetric distribution to daughter cells. Monitoring histone H2B-GFP also permits continuous analysis of chromosomal degradation during apoptosis.

Quality

Each vial contains ≥ 1X10⁶ viable cells.
Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
Cells are negative for mycoplasma contamination.
Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.

Storage and Stability

Histone H2B-GFP expressing HeLa Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.