Analysis Note

Molecular weight based on viscosity. Also assayed by MALLS.

Application

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm². Optimal conditions for attachment must be determined for each cell line and application.Poly-L-lysine hydrobromide has been used to adhere the dissociated cells of cortical tissues into the culture plates. It has also been used to treat slides, to fix samples on it for immunohistochemical analysis.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

General description

Poly-L-lysine hydrobromide is a nonspecific attachment factor in promoting cell adhesion to the substratum.

Other Notes

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.