Application

Polyethylene glycol solution has been used in the hybridization of the following:irradiated camel fibroblast cells with A23 hamster cellsmouse splenocytes with Sp2/0-Ag 14 mouse myeloma cellsrabbit splenocytes and cell line 240E-1

Recommended for use in a normal fusion protocol requiring 50% PEG.

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.

Biochem/physiol Actions

Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics. PEG is commonly used as a lubricant. It′s application is also observed in chromatographic stationary phases. PEG prevents crystal formation in emulsions.

General description

Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Other Notes

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.

Packaging

Packaged in sealed ampules under nitrogen.

Reconstitution

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.