Primate embryonic stem cells and induced-pluripotent stem cells are extremely sensitive to cryopreservation which presents many difficulties when compared to murine or other cells. At present, the vitrification method is considered adequate for primate ES/iPS cells although the slow-freezing method using DMSO has been popular for a wide variety of cell lines. Vitrification is the rapid cooling of the freezing media to a glass-like crystalline state. The vitrification method requires impeccable timing, a high level of skill, and still can yield poor results. In addition, this method shows sensitivity to dry ice transportation. To address these problems, the new freezing medium Bambanker™ hRM has been developed. The cell freezing media Bambanker™ hRM contains no bovine serum albumin, which can sometimes lead to cell differentiation. Furthermore, there are no animal-derived material which improve storage and survivability of primate ES/iPS cells while greatly simplifying the protocol.
Serum adds variation to long-term storage: All Bambanker™ products are produced with no serum. Cryopreservation media which contain sera have the disadvantage of fluctuations in recovery rates and undefined composition. Reproducibility of experiments with cells which were frozen in a serum-containing-medium, could be affected by lot-to-lot variation of the serum since the composition and concentration of proteins and other biological molecules varies with each batch of serum. This may result in issues when thawing and using cells from such serum-containing media. Breathe easy, as every ingredient of Bambanker™ is precisely defined so that you can be confident that cells stored at different times will all behave and recover similarly.
Highest GMP quality: All products of the Bambanker™ series are manufactured in facilities compliant with current good manufacturing practices (GMP). The products are subject to a solid quality management system that ensures the consistency, reliability and high quality you can rely on.
There are four different versions of Bambanker™. All Bambanker™ types are free of fetal bovine serum (FBS) and have a defined and constant composition. They also do not contain antibiotic or antifungal ingredients. The four different types of Bambanker™ are recommended depending on your specific needs:
- Bambanker™: standard for all cell lines
- Bambanker™ Direct: for hybridoma cells
- Bambanker™ DMSO Free: for high sensitivity cells
- Bambanker™ hRM: for ES and iPS cells
Features:
- Optimal for the cryopreservation of primate ES and iPS cells
- Made with human serum albumin – no other animal components
- Enables long term cell storage at -80 or -196°C
- Improved cell recovery and viability after thawing
- Ready-to-use – no programmed or sequential freezing required
- Serum-free – no risk of contamination
- Produced in a GMP compliant facility
- Usable for all known cell lines
- Long shelf life
This product has met the following criteria to qualify for the following awards: