Cryopreservation of mammalian cells is extremely valuable and common in biological research. Fear of losing a cell line to contamination or incubator failure is frequently the impetus for making archival storage of cells a high priority after receiving or generating a new cell line. Once transferred from growth media to freezing medium, the cells are usually frozen at a controlled rate and stored in liquid nitrogen vapor or at -130°C in a mechanical deep freeze. Although freezing a cell line is a commonly performed procedure, problems arise when suitable freezers are not available, or unwanted variables are introduced by the presence of serum, extra-wash or complicated freezing algorithms. The cell freezing media Bambanker™ permits cryopreservation of cells at -80°C (or in liquid nitrogen), obviating the need for an additional and expensive ultra-low freezer and avoiding time consuming and complicated controlled freezing protocols. Simply (1) harvest cells, (2) aspirate medium, (3) resuspend in Bambanker™ (4) transfer to a cryovial and (5) store at -80°C. No programmed or sequential freezing is required! Bambanker™ is a serum-free cryopreservation medium that is delivered ready-to-use.
Serum adds variation to long-term storage: All Bambanker™ products are produced with no serum. Cryopreservation media which contain sera have the disadvantage of fluctuations in recovery rates and undefined composition. Reproducibility of experiments with cells which were frozen in a serum-containing-medium, could be affected by lot-to-lot variation of the serum since the composition and concentration of proteins and other biological molecules varies with each batch of serum. This may result in issues when thawing and using cells from such serum-containing media. Breathe easy, as every ingredient of Bambanker™ is precisely defined so that you can be confident that cells stored at different times will all behave and recover similarly.
Universal – even for preservation of stem cells: All common cell lines can be preserved resulting in a high number of intact cells after thawing. Recovery rates of even sensitive cells is much higher compared to regular media. For example the JCRB cell bank stores over 1,400 different cell lines with great success with Bambanker™.
Highest GMP quality: All products of the Bambanker™ series are manufactured in facilities compliant with current good manufacturing practices (GMP). The products are subject to a solid quality management system that ensures the consistency, reliability and high quality you can rely on.
There are four different versions of Bambanker™. All Bambanker™ types are free of fetal bovine serum (FBS) and have a defined and constant composition. They also do not contain antibiotic or antifungal ingredients. The four different types of Bambanker™ are recommended depending on your specific needs:
- Bambanker™: standard for all cell lines
- Bambanker™ Direct: for hybridoma cells
- Bambanker™ DMSO Free: for high sensitivity cells
- Bambanker™ hRM: for ES and iPS cells
Features:
- Enables long term cell storage at -80 or -196°C
- Improved cell recovery and viability after thawing
- Ready-to-use – no programmed or sequential freezing required
- Serum-free – no risk of contamination
- Produced in a GMP compliant facility
- Usable for all known cell lines
- Long shelf life
- Highest cell viability and recovery ﹥90% – keep your cells safe
- Quick and easy protocol – just add and freeze
This product has met the following criteria to qualify for the following awards: