Application

Anti-NANOG Antibody, clone 7F7.1, Alexa Fluor 488 conjugate.

General description

NANOG (Homeobox protein NANOG) is a member of the Nanog homeobox family of DNA-binding proteins. It is expressed in embryonic stem cells and confers pluripotency on these cells. Once embryonic stem cells become differentiated, NANOG expression is suppressed. NANOG is involved in the Hedgehog/Gli1 signaling pathway which has been implicated in the development and growth of various types of tumors. NANOG has also been identified as a key transcription factor used to generate induced pluripotent stem cells.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG2a conjugated to Alexa Fluor^® 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Quality

Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected NANOG in H9 human embryonic stem cells.

Target description

The uncojugated parent antibody (Catalog No. MABD24) has an observed MW of 39 kDa