Application

Evaluated by Immunocytochemistry on human bone marrow mesenchymal stem cells. Immunocytochemical analysis: A 1:50 dilutions of this antibody detected CD73 on 1X10^6 human adipose and bone marrow mesenchymal stem cells.

Research CategoryStem Cell Research

Research Sub CategoryMesenchymal Stem Cells

This Anti-CD73, clone AA60-E3-3 is validated for use in Immunocytochemistry for the detection of CD73.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CD73, also known as Ecto-5′-nucleotidase, catalyzes the conversion of purine 5′ mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation, being expressed on subsets of B-cells and T-cells. CD73 is also a marker of undifferentiated mesenchymal stem cells and is often used to identified stem cell populations.

Immunogen

Whole Mouse Dermal Lymphatic Endothelial Cells (DLEC)

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1κ conjugated to Alexa Fluor^® 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Quality

Evaluated by Immunocytochemistry on human adipose mesenchymal stem cells.
Immunocytochemical analysis: A 1:50 dilutions of this antibody detected CD73 on 1X10^6 human adipose and bone marrow mesenchymal stem cells.

Storage and Stability

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

The unconjugated parent antibody (Catalog No. MABD122) has an observed MW at 63 kDa