Analysis Note

ControlA431 human epidermoid cancer cells.

Application

Evaluated by Immunocytochemistry in human bone marrow mesenchymal stem cells (SCR108). Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected nuclei in human bone marrow mesenchymal stem cells (SCR108). Evaluated by Immunocytochemistry in primary mouse embryonic fibroblasts (PMEFs). Immunocytochemistry Analysis: A 1:200 dilution of this antibody did not detect nuclei in primary mouse embryonic fibroblasts (PMEFs).

Research CategoryStem Cell ResearchNeuroscience

Research Sub CategoryNeuroregenerative MedicineNeural Stem Cell Migration

Detect Nuclei Marker using this mouse monoclonal antibody, Anti-Human Nuclei Antibody, clone 235-1 & Biotin Conjugate validated for use in ICC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models. MAB1281 stains nuclei of all human and primate cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. The antibody does not react with nuclei from mouse or rat.

Immunogen

Human nuclei

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15mg/ml BSA.

Quality

Evaluated by Immunocytochemistry in A431 human epidermoid cancer cells. Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected nuclei in A431 human epidermoid cancer cells.

Specificity

This antibody recognizes human and primate nuclei. Antibody is not reactive with rat or mouse nuclei.

Storage and Stability

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.