Analysis Note

ControlCOS7 cells.

Application

Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1 is an antibody against Mitochondria for use in IF, IH, IH(P) & IP.

Immunohistochemistry:
1:100 dilutoin from a previous lot was used. Works best with organic fixatives (acetone, methanol, etc). 2% PFA for 10-15 min can be used but stronger use of formalin or 4% PFA limits staining.
The antibody has been used to isolate intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads {http://www.dynalbiotech.com}.

Immunohistochemistry in paraffin embedded tissues:
Requires citric acid/microwave antigen retrieval; enhanced ABC or amplified detection systems recommended.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Heart Ventricle Epithelium

The antibody has been used to isolate intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads {http://www.dynalbiotech.com}.

Indirect immunofluorescence:
1:10-1:50 dilution from a previous lot was used, 30 - 100 μL per slide well.

MAB1273 recognizes a 65 kD protein by immunoprecipitation.

Optimal working dilutions and protocols must be determined by the end user.

General description

This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions.

Immunogen

Epitope: surface of intact mitochondria

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1 in buffer containing liquid in PBS. Contains no preservative. Concentrated hybridoma supernatant.

Format: Purified

Quality

Routinely evaluated by immunohistochemistry on heart ventricle cells.

Immunohistochemistry(paraffin) Analysis:
Mitochondria (cat. # MAB1273) staining on heart ventricle cells. Tissue pretreated with EDTA, pH 8.0This lot of antibody was diluted to 1:80, using IHC-Select™ Detection with HRP-DAB. Immunoreactivity is seeing the circular-granular layer surrounding the nucleus of the cells.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Heart Ventricle Epithelium

Specificity

Reactivity with other species has not been determined

Gives mitochondrial staining on all human cell types and does not cross react with rat and mouse tissue. The staining pattern is best described as a spaghetti-like staining pattern in fibroblasts and a perinuclear large speckled pattern in lymphonoid cells.
MAB1273 recognizes a 65 kD protein by immunoprecipitation. Antibody produces the stringy, "spaghetti-like" staining patten in the cytoplasm of human cells.

Storage and Stability

Stable for 6 months at -20°C in undiluted aliquots from date of receipt.

Target description

65 kDa