Application

Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.

Methotrexate hydrate has been used: as a dihydrofolate (DHFR)inhibitor in sphere forming assay cultureas a component of dulbecco′ modified Eagle′s medium (DMEM) for selection of transfected cells in viability and co-culture assays

Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.

Biochem/physiol Actions

Methotrexate used to inhibit dihyrofolate reductase and select for DHFR overexpresssion.

Methotrexate is a folic acid antagonist.