Application

Insulin-transferrin-sodium selenite, linoleic;oleic-BSA is used for serum-free cell culture applications.

Components

Contains 1.0 mg/ml bovine insulin, 0.55 mg/ml human transferrin (substantially iron-free), 0.5 µg/ml sodium selenite, 470 µg/ml linoleic acid, 470 µg/ml oleic acid and 50 mg/ml bovine serum albumin at the 100x concentration. Contains 2 moles each of linoleic acid and oleic acid per mole of albumin.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.