Application

Insulin-transferrin-sodium selenite, linoleic-BSA has been used for serum-free cell culture applications.ITS+1 Liquid Media Supplement (100×) has been used in culturing cell lines and for the preparation of immortalisation medium for the production of cell lines from ′Immortomouse′ embryos.

Components

Contains 1.0 mg/ml bovine insulin, 0.55 mg/ml human transferrin (substantially iron-free), 0.5 µg/ml sodium selenite, 50 mg/ml bovine serum albumin and 470 µg/ml linoleic acid at the 100x concentration.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.