Application

HEPES has been used as a component of:Hank’;s balanced salt solution, Dulbecco’;s modified eagle’;s medium and no-glucose DMEM, which are used for the preparation of tissue slicesBuffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity

Caution

Concentrations of HEPES over 40mM may be toxic to some cell types.

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Preparation Note

Prepared from HEPES free acid and cell culture grade water.