Application

Research CategoryStem Cell Research

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

BAFF, a member of the TNF superfamily of ligands, is expressed in T cells, macrophages, monocytes and
dendritic cells. BAFF is involved in stimulation of B and T cell function, and is an important survival and maturation factor for peripheral B cells. BAFF signals through three different TNF receptors TACI, BCMA and BAFF-R.
Recombinant human soluble BAFF is a 152 amino acid polypeptide (17.0 kDa), which contains the TNF-like portion of the extracellular domain of BAFF.

Product Source: Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.

Physical form

Product is presented in 20 mM Tris, pH 8.0 + 215mM NaCl and is filtered through a 0.2 micron filter before lyophilization.

Quality

Bioactivity assay: Determined by a mouse splenocyte
survival assay. The expected ED50 for this effect is 0.5-2.0
µg/ml.

Storage and Stability

Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.