Application

L-Glutamine has been used as a media supplement:for cochlear organotypic culturingin Iscove′s modified Dulbecco′s medium for HeLa cells culturingin Dulbecco′s modified Eagle′s medium for primary hepatocyte cell culturing

Biochem/physiol Actions

Glutamine mediates protein turnover via cellular mTOR (mammalian target of rapamycin) signalling. It is also known to be associated with the inhibition of apoptosis.

L-glutamine is an essential amino acid that is a crucial component of culture media, and serves as a major energy source for cells in culture. Optimal cell performance usually requires supplementation of the medium with L-glutamine prior to use.

L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Other Notes

The dipeptide L-Ala-LGln is frequently used in modern serum-free media formulations because it provides a more stable form of glutamine in vitro.

Packaging

50 mL amber serum vial with rubber stopper.

Reconstitution

Prepares a 200 mM solution when reconstituted to 10 mL with sterile water.

Suitability

Qualified for use in cell culture applications, especially serum-free cell culture.