Analysis Note

Controlmouse ES cells (CMTI-2)

Application

Milli-Mark Anti-Brachyury-FITC Antibody, clone 3E4.2 is an antibody against Brachyury for use in FC.

Research CategoryStem Cell Research

Research Sub CategoryMesenchymal Stem Cells

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Brachyury protein is a T-box transcription factor that is one of the earliest indicators of mesoderm formation during embryonic development. As the brachyury protein family is essential for the formation of the posterior body in all vertebrates, Brachyury has been shown to be expressed during gastrula and neurula stages, as well as in the developing notochord. Consequently, when mutations occur within the brachyury gene, severe vertebral malformations have been found to occur, as a direct result. In addition, current findings also indicate that the dysregulation of brachyury may also be linked to the formation of chordomas, which are malignant tumors that develop along the spine.

Immunogen

Synthetic human brachyury peptide, a.a. 224-435.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified mouse monoclonal IgG1k conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Protein G Purified

Quality

Evaluated by flow cytometry using mouse ES cells (CMTI-2)

Specificity

Antibody recognizes Brachyury.

Storage and Stability

Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.

Target description

47 kDa Calculated