Analysis Note

Control2102 Ep Cells.

Application

Research CategoryStem Cell Research

This Milli-Mark Anti-SSEA3 Alexa 488 Antibody, clone MC-631 is validated for use in FC for the detection of SSEA3 Alexa 488.

Research Sub CategoryPluripotent & Early Differentiation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The cell-surface markers used routinely to identify undifferentiated human embryonic stem cells (hESCs) were initially characterized as markers for mESCs, mouse embryonic carcinomas (ECs), or human EC cells. Stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 are widely used as cell-surface markers to define both human and mouse ESCs. SSEA-3 and SSEA-4 are expressed on undifferentiated hESCs but not on undifferentiated mESCs.

Immunogen

4-8 cell stage mouse embryos

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Unconjugated antibody Cat. No. MAB4303-I is known to react with mouse.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgM conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Purified by Ion-Exchange Chromatography, prior to conjugation with Alexa488.

Quality

Evaluated by flow cytometry using 2102 Ep cells.

Specificity

Reacts with the Stage-specific embryonic antigen-3 (SSEA-3) that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES).

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.