Analysis Note
Control2102 Ep cells.
Application
Research CategoryStem Cell Research
| Working Solution | Cells / test | Total Reaction Volume |
|---|
| 5 µl | 5 x 105 Cells / 95 µl PBS | 100 µl |
| 10 µl | 1 x 106 Cells / 90 µl PBS | 100 µl |
Detect TRA-2-49 using this Milli-Mark Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate validated for use in FC.
Research Sub CategoryPluripotent & Early Differentiation
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.
Immunogen
Epitope: Unknown
2102Ep human Embryonal Carcinoma Cells
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form
Protein A purified
Purified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality
Evaluated by flow cytometry using 2102 Ep cells.
Specificity
Antibody recognizes TRA-2-49.
Storage and Stability
Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Target description
212/80/55 kDa Observed
This product has met the following criteria to qualify for the following awards: