4,6-Diamidino-2-phenylindole dihydrochloride, powder, BioReagent, suitable for cell culture, >=98% (HPLC and TLC), suitable for fluorescence

Code: D8417-5MG D2-231

Application

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probe...


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€187.00 5MG
€230.01 inc. VAT
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Application

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

4′,6-Diamidino-2-phenylindole dihydrochloride has been used in staining of various cells in immunocytochemistry and cytochemistry, human subcutaneous adipose tissues for fluorescence imaging, embryo sections for immunofluorescence.

Biochem/physiol Actions

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Caution

Protect from light.

General description

4′,6-Diamidino-2-phenylindole dihydrochloride is a fluorescent dye for staining DNA. It strongly interacts with the dAT-rich (deoxy adenine-thymine) regions of the DNA.

Packaging

1, 5, 10 mg in glass bottle

ε (extinction coefficient)30 at 263 nm in H2O
assay≥98% (HPLC and TLC)
fluorescenceλex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 340 nm; λem 488 nm (nur DAPI)
formpowder
InChI keyFPNZBYLXNYPRLR-UHFFFAOYSA-N
InChI1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
product lineBioReagent
Quality Level200
SMILES stringCl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
solubilityPBS: insoluble, H2O: 20 mg/mL (Heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)
storage temp.2-8°C
suitabilitysuitable for fluorescence
technique(s)cell culture | mammalian: suitable
Cas Number28718-90-3
Pack5MG
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