Application

Supports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.

Research CategoryStem Cell ResearchCell StructureECM & Adhesion

General description

PluriSTEM™ Recombinant Vitronectin is an effective xeno-free alternative to matrigel™ and has been validated to successully culture human ES and iPS cells using PluriSTEM™ Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.

PluriSTEM™ Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.

Legal Information

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Product is sterile-filtered in a 20 mM pH 8.0 Tris-HCl buffer, with a proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and glycerol.

Quality

Each lot is tested on human ES cells (H1) cultured using 5-10 µg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate).

Storage and Stability

Store at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles.