Analysis Note

ControlDax1 overexpressing HEK293 transfected cell line

Application

Research CategoryStem Cell Research

This Dax1 antibody is validated for use in WB & IHC for the detection of the Dax1 protein.

Research Sub CategoryPluripotent & Early Differentiation

Evaluated by Immunohistochemistry in Human and Rat adrenal gland tissue. Immunohistochemsitry Analysis: A 1:1000 dilution of this antibody detected Dax1 in Human and Rat glioblastoma adrenal gland tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Dax-1 is an orphan nuclear receptor and is required for the normal development of the hypothalamic-pituitary-adrenal-gonadal axis formation. Defects in Dax-1 are the cause of X-linked adrenal hypoplasia congenital (XL-AHC); also known as X-linked Addison disease (AHX). Dax-1 also has a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency by acting as a negative regulator of Oct3/4 in ES cells.

Immunogen

KLH-conjugated linear peptide corresponding to the Region of Ligand binding of Human Dax1.

Linkage

Replaces: 04-445

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in a Dax1 overexpressing HEK293 transfected cell line. Western Blot Analysis: A 1:2000 dilution of this antibody detected Dax1 in a Dax1 overexpressing HEK293 transfected cell line.

Specificity

75% Homologogous to Mouse Dax1

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~53 kDa observed