Aminopterin, Hybri-Max, 50 x, gamma-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

Code: A5159-10VL D2-231

Application

Aminopterin concentrate supplement for hybridoma cell culture applications.

Biochem/physiol Actions

Folic acid antagonist. Aminopteri...


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Application

Aminopterin concentrate supplement for hybridoma cell culture applications.

Biochem/physiol Actions

Folic acid antagonist. Aminopterin is actively transported into cells by the folate transporter. In the cell, it is converted to a high molecular weight polyglutamate metabolite by folylpolyglutamate synthase that, in turn, binds to dihydrofolate reductase and inhibits its activity. Aminopterin-polyglutamate is degraded intracellularly by γ-glutamyl hydrolase.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Linkage

More potent, but more toxic, than methotrexate.

Other Notes

Tandem Mass Spectrometry data independently generated by Scripps Center for Metabolomics is available to view or download in PDF. A5159.pdf Tested metabolites are featured on Scripps Center for Metabolomics METLIN Metabolite Database. To learn more, visit sigma.com/metlin.

Reconstitution

Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500 mL medium. Final working concentration: 0.4 µM aminopterin.

ε (extinction coefficient)25,700 at 261 nm in 0.1 M NaOH at 1 M, 8,100 at 373 nm in 0.1 M NaOH at 1 M, 24,500 at 282 nm in 0.1 M NaOH at 1 M
biological sourcesynthetic
concentration50 ×
formlyophilized powder
Gene Informationhuman ... FPGS(2356)mouse ... Fpgs(14287)
gradeHybri-Max™
impuritiesendotoxin, tested
InChI keyTVZGACDUOSZQKY-LBPRGKRZSA-N
InChI1S/C19H20N8O5/c20-15-14-16(27-19(21)26-15)23-8-11(24-14)7-22-10-3-1-9(2-4-10)17(30)25-12(18(31)32)5-6-13(28)29/h1-4,8,12,22H,5-7H2,(H,25,30)(H,28,29)(H,31,32)(H4,20,21,23,26,27)/t12-/m0/s1
product lineBioXtra
Quality Level200
shipped indry ice
SMILES stringNc1nc(N)c2nc(CNc3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O)cnc2n1
solubility10 mL/vial, clear, red (in TC Medium)
sterilityγ-irradiated
storage temp.−20°C
technique(s)cell culture | hybridoma: suitable
Cas Number54-62-6
Hazard Class6.1
Un Number2811
Pack GroupI
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